肠炎沙门氏菌
沙门氏菌
代谢物
代谢组学
食品科学
生肉
化学
生物
细菌
色谱法
生物化学
遗传学
作者
Lan Chen,Hao Ding,Yali Zhu,Yawen Guo,Yayun Tang,Kaizhou Xie,Genxi Zhang,Guojun Dai,Yushi Gao,Tao Zhang
出处
期刊:Food Chemistry
[Elsevier]
日期:2023-05-01
卷期号:409: 135294-135294
被引量:12
标识
DOI:10.1016/j.foodchem.2022.135294
摘要
Salmonella Enteritidis easily contaminate chicken during slaughtering, processing, transportation, and sales, which seriously endangers human health. This study aimed to identify metabolite biomarkers for Salmonella Enteritidis contamination in chicken meat. UPLC-Q-Orbitrap MS untargeted metabolomics analysis identified 441 and 240 confidently metabolites in positive and negative ion mode, respectively. Thirty metabolites were defined as potential biomarkers for Salmonella enteritidis contamination in chicken meat. UPLC-QQQ-MS based targeted metabolomics was used to quantitatively analyze candidate metabolite biomarkers in Salmonella enteritidis contaminated and fresh chicken samples. A total of 10 candidate metabolite biomarkers were confirmed in the validation set, among which acetylcholine, l-Methionine, l-Proline, l-Valine, and l-Norleucine were identified as biomarkers for Salmonella Enteritidis contamination in chicken. The combined receiver operating characteristic curve analysis of the five biomarkers achieved an AUC of 0.956, indicating their high sensitivity and specificity in predicting Salmonella Enteritidis in raw chicken. In conclusion, the present study identified five metabolite biomarkers for Salmonella enteritidis in raw chicken. These results provide a potential theoretical basis for developing Salmonella Enteritidis detection methods in raw chicken.
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