A fluorescence probe with targeted mitochondria was developed for detecting H2O2 in vitro and vivo

We designed and developed the probe P-1 for detecting H2O2. The probe can selectively detect H2O2 with colorimetric change. In addition, the combination of portable test strips with a smartphone platform provided great convenience for on-site and visual detection of H2O2 with satisfactory sensitivity and reliability. The P-1 can image exogenous and endogenous H2O2 in cells and distinguish cancer cells and normal cells by fluorescence image of H2O2 and flow cytometry. Meanwhile, the P-1 probe has been successfully applied to monitor H2O2 levels of tumor in mice, and the results showed the level of H2O2 in tumor tissue increased significantly. Therefore, P-1 provided a potential chemical tool to understand pathological and physiological mechanisms of diseases by imaging H2O2.