The correlation and path analysis as well as grey correlation analysis of ten physiology and biochemistry indices and changes of fruit firmness were studied with winter jujube during low temperature storage. The study showed that Vc content, organic acid content and rate of water loss have larger influence, which are the dominant factor of impact on firmness. For this reason, Vc content, organic acid content and rate of water loss can be the reference when selecting winter jujube storage and transportation material.