The experiment was designed to investigate effects of 37℃ constant heat stress on production efficiency in broiler chickens with experimental colibacillosis and to give the theoretics basis for breeding broiler chickens with colibacillsis under high temperature.The results showed that: the heat stress and colibacillosis in broiler chickens all decreased significantly(P0.01) in feed intake.The feed intake of broiler chickens with colibacillsis decreased by 26.01%(P0.01) from 4 to 7 day under heat stress.The heat stress decreased significantly(P0.01) in body weight gain of broiler chickers.The body weight of broiler chickens with colibacillosis decreased significantly(P0.01).The body weight in broiler chickens with colibacillosis decreased by 348.58% in 7 days under heat stress.The conclusion: The heat stress decreased significantly in feed intake of broiler chickens with colibacillosis and severely affected its production efficiency.