Anti-proliferative effects of Semen Sojae Praeparatum and Black Soybean extract and the content determination of four isoflavone components

Objective: To investigate the effect of Semen Sojae Praeparatum(SSP) and black soybean(BS) extracts on the MCF-7 cell proliferation,and determine of four isoflavone components content.Methods: MTT method was used to observe the effect of SSP and BS extract on the proliferation of breast cancer cell line MCF-7.Early cells apoptosis were detected by Annexin/PI flow cytometry analysis.Four isoflavones content(daidzin,genistin,daidzein and genistein) from SSP and BS extract were analyzed with HPLC assay.Results: Extracts of SSP and BS inhibited cell proliferation and induced apoptosis in MCF-7cancer cells.However,the high concentration of SSP extract resulted in a significantly inhibition than BS in cancer cell proliferation(P0.05).Daidzin,daidzein and genistein in SSP extract were significantly higher than that of BS extract(P0.01),but there is no change in genistin.Conclusion: SSP extract has strong inhibition of cancer cell proliferation compared with BS extract.Higher levels of daidzein,daidzin and genistein in SSP extract may be the mechanism for stronger effect of anti-cancer cell proliferation.