Analysis of the Genetic Relationships of Main Auricularia auricular Cultispecies in Qinba Mountainous Area Based on ITS Sequence

Analyzing the genetic relationships of the main Auricularia auricular cultispecies in Qinba mountainous area by ITS sequence so as to lay a theoretical foundation to genetics and breeding. Using CTAB method to extract genomic DNA from the bacterial strain,Auricularia auricular,and amplifying and sequencing PCR products;Auricularia auricula-judae 2 and Auricularia auricula-judae HF-8(GQ168492) were clustered in the same branch,Auricularia auricula-judae 913 and Auricularia auricula-judae YBS-7(GQ168503) were clustered in the same branch,but the genetic relationship between Auricularia auricula-judae Shennong A8 and the Auricularia auricula-judae 2,Auricularia auricula-judae 913 were relatively alienative. Auricularia auricula-judae 97-2,Auricularia auricula-judae NW482(EU520194),the Auricularia auricula-judae 268 and Auricularia auricula-judae NW491(EU520160) were clustered in the same branch.However the genetic relationships among Auricularia auricula-judae Qindan 4,Auricularia auricula-judae 97-2,Auricularia auricula-judae 268 were relatively alienative. The genetic relationships among Auricularia auricula-judae Xinke,Auricularia auricula-judae 2,Auricularia auricula-judae 913 and Auricularia auricula-judae Shennong A8 were close. However,the relationships between Auricularia auricula-judae 268,Auricularia auricula-judae Qindan 4#and Auricularia auricula-judae 97-2 were quite far. Through the morphologic and ITS analysis of Auricularia auricular strains,two dominant strains,Auricularia auricula-judae Qindan 4#and Auricularia auricula-judae Shennong A8,have alienative relationship. Our findings established a solid foundation to genetics and breeding.