Inhibitory effects of 14 kinds of Chinese herbal medicine against enterogenic probiotics and enterohemorrhage E. coli

Active ingredients of 14 kinds of Chinese herbal medicine were extracted using decoction method,and their inhibitory effects on four enterogenic probiotics strains and one enterohemorrhage E.coli(EHEC) strain were tested with disc diffusion method,respectively.The results showed that Ligusticum wallichii Franch,Scutellaria baicalensis Georl,Isatidis Radix,Rheum officinale Baill and Glycyrrhiza uralensis Fisch had high antibacterial effects on the four enterogenic probiotics strains,and the bacteriostatic diameter was 22 mm;Pwraria lobata(Willd) Ohwi,Pericarpium Citri Reticulatae,Andrographis paniculata(Burm.f.)Ness and Atractylodes chinensis(DC.) Koidz.had lower antibacterial abilities,the bacteriostatic diameter was 11 mm,and the others had no antibacterial ability;Scutellaria baicalensis Georgl,Astragalus membranaceus(Fisch.) Bunge,Acorus gramineus Soland and Pericarpium Citri Reticulatee,Glycyrrhiza uralensis Fisch.Atractylodes chinensis(DC.)Koidz had antibacterial effects on EHEC strain, the bacteriostatic diameter was 10-24 mm, Scutellaria baicalensis Georgl had the biggest significant effect of inhibition on EHEC.MICof Astragalus membranaceus(F ish.) Bunge to EHEC were 0.625g /m L, less than the four kinds of probiotics, Ligusticum wallichii Franch, Pueraria lobata(W illd.) Ohwi, Paeonia suffruticosa Andr, Isatidis Radix L., Anemarrhena asphodeloides Bunge, Andrographis paniculata(B urm.f.) Nees, Rheum officinale Baill and Poria cocos(S chw.) Wolf had no inhibition on EHEC strain.