Effects of ionizing radiation on IL-12 and NF-κB activities in peritoneal macrophages of mice

Objective To observe the changes of IL\|12 and NF κB in murine peritoneal macrophages after whole\|body irradiation(WBI) with different doses X\|rays. Methods Enzyme\|linked immunosorbent assay(ELISA) and electrophoresis mobility shift assay(EMSA) were used to detect the secretion of IL\|12 and the DNA\|binding activity of NF κB,respectively. Results ELISA data indicated both 0 075 Gy and 2 Gy increased the secretion of IL\|12 and the latter seemed to increase IL\|12 more greater than the former.EMSA showed two bands representing P65/P50 and P50/P50 respectively. 0 075 Gy increased the P65/P50 activity and showed little effect on P50/P50,while 2 Gy increased both the two dimmers,consequently elevated the DNA\|binding activity of NF κB more significantly. Conclusion The results suggested that NF κB might participate in the radiation\|induced changes of IL\|12.\;