The purpose of this study was to determine optimum condition for the cultivation of Chlorella sp. FC-21, which is a freshwater microalgae, using light emitting diodes (LEDs). Specific growth rate and cell concentration were measured for the reactors at the illumination of different wavelengths of LEDs. Among various types of LEDs, red LEDs were the most effective light source, and also greatest increases of specific growth rate and cell concentrations were obtained when light intensity of red LEDs increased. From this study, we found that red LEDs were the most appropriate light source for the cultivation of Chlorella sp. FC-21.