Effect of Different Culture Conditions on Cell Growth in Suspension Culture of L.heterophyllus

Callus of Leonurus heterophyllus Sw.from solid medium were cultured in liquid medium,and the types of basal medium,concentration of plant growth regulators,content of sucrose in medium,and quantity of original incubated callus were respectively tested on the increase of callus proliferation.It was found that MS medium was most suitable for suspended cell growth,and the biomass of the cells increased rapidly in medium with 3% sucrose.6g/L original incubated callus showed the most promptional effect on callus growth, however the cells stopped to growth ifvery small callus incubated in medium.In medium with combination of 6-BA(2 mg/L) and 2,4-D(0.5 rog/L) or NAA(0.5 mg/L),suspended cells proliferated most rapidly.The results presented here provide the consults for massive culture of L.heterophyllus and extraction and utilization of alkloid for this plant.