Preparation and optimization of temozolomide liposome

OBJECTIVE To prepare temozolomide liposomes.METHODS The temozolomide liposomes were prepared with phospholipid and cholesterol.To separate the liposomes and free drugs with high speed centrifugation.The entrapment efficiency was determined by ultraviolet spectrophotometry.To investigate the effects to the temozolomide liposomes of the prepration methods and technology on the basis of entrapment efficiency.RESULTS The entrapment efficiency of temozolomide liposomes prepared by the antiphase evaporation method was (33.6±3.8)%.CONCLUSION It is hard to raise the entrapment efficiency of temozolomide liposomes because of the limitation of the temozolomide′s dissolvability.