Improvement on culture in vitro of rat cerebral cortical astrocytes

Objective To establish a culture method of rat cerebral cortical astrocyte for further research on astrocyte in vitro.Methods Cerebral cortex of postnatal 5 days of SD rats were used basing on McCarthy method.Unicell suspension were prepared and inoculated in culture flask.Changed the culture flask after 1 hour and substratum was replaced after 3 days.Passage was done when cells syncretized to monolayer.Glial fibrillary acidic protein of the specifical sign of astrocyte was monitored by indirect immunofluorescence after three times passage.The masccline cells of glial fibrillary acidic protein were affirmed to be astrocyte.Results All cerebral cortical astrocytes cells which were cultured in vitro underwent adherence,fibroblast removed,depuration and passage three times,the rate of masccline cells of glial fibrillary acidic protein was above 95%.Conclusion Culture of cerebral cortical astrocytes in vitro is successful and there is higher purity.The condition was provided for further research of astrocytes.