Effect of Toutongning Capsule on P2X3mRNA Expression in Trigeminal Ganglion of Migraine Rats

Objective:To observe the effects of Toutongning capsule on P2X3mRNA expression in trigeminal ganglion of migraine rats.Method:The healthy SD rats were evenly divided into:normal group.model group,three dose groups of Toutongning(760,380,190 mg·kg-1)capsule group and Xibiling group.Migrained rats model was established by NTG subcutaneous injection.Samples were harvested and measured in 3 hours and 6 hours after NTG subcutaneous injection.The P2X3mRNA expression in trigeminal ganglion was determined by RT-PCR.Result: P2X3mRNA expression in trigeminal ganglion increased in the model group compared with that in the normal group(P0.01).P2X3mRNA expression was elevated at hour 3 then decreased at hour 6.P2X3mRNA expression in trigeminal ganglion and thalamencephalon were downregulated in the three dose groups of Toutongning capsule and Xibiling group compared with those in the model group(P0.01).Conclusion: Toutongning capsule could treat migraine by reducing P2X3mRNA expression in trigeminal ganglion.