COMPLETE GENOMIC SEQUENCE ANALYSIS OF A SHANGHAI LSOLATE OF CANINE PARVOVIRUS

To study the genomic organization and genotype of a Shanghai isolate of Canine parvovirus(CPV-SH),primers were designed on the basis of the complete genomic sequence of 3 Canine parvovirus serotypes in the GenBank,and the genomic sequence was amplified from the isolate by PCR.The PCR products were cloned into T vector and sequenced.Phylogenetic tree was constructed by the Neighbor-joining method with Mega 4 software.The complete genomic sequence of CPV-SH was 4 758 bp in length.The full genome sequence was deposited in GenBank under access no.FJ792845.Comparison of this sequence with that of VP2 gene of 3 Canine parvovirus serotypes in GenBank revealed high homology with genotypes 2a,2,2b and 2c(99.7%,98.8%,99.5%,and 99.5%).Genetic analysis of 20 other Chinese isolates also found that CPV-SH belonged to genotype 2a and shared 99.9% homology with a Beijing isolate(CPV/BJ082).