Microsatellite enrichment by magnetic beads in Coelomactra antiquate

In this experiment,magnetic bead hybridization method was used to enrich the microsatellite loci in Coelomactra antiquate.As a result,38 microsatellites that repeated no less than 5 times were found.Among the 38 microsatellites,68.4% are perfect,23.7% are imperfect and the rest are compound(7.9%).In addition to the biotin-labeled SRS(simple repetitive sequence) probe CA and GA,there are ATTG,TG,CGTG and TGTTG in the microsatellites.Microsatellite repeats mainly from 5 to 46 times,and the most repetitive number is 70.We designed 14 pairs of SSR-primers using these microsatellite sites,which will facilitate the studies on the genetic diversity of C.antiquata.