In this study, antioxidant peptides from crab were prepared through enzymatic hydrolysis using swimming carb waste as the material. Antioxidant activity of hydrolysates was evaluated using reducing power assays. Results indicated that neutral protease was suitable for the hydrolysis of swimming crab waste. The optimal hydrolysis condition was 1:6 of materialliquid ratio, 1.5 h of water-bath heating treatment, pH 7.5, 3000 U/g of enzyme amount and 4 h of hydrolysis. The highest reducing power was determined in hydrolysates obtained under the optimal hydrolysis condition and the content of polypeptides in hydrolysates was 65.66%.