Identification for enzymatic degradation products of Asini Corii Colla by Tricine-SDS-PAGE

AIM To use Tricine-SDS-PAGE method to analyze enzymatic degradation products of Asini Corii Colla.METHODS Pepsin,trypsin,chymotrypsin and papain were respectively employed to degrade Asini Corii Colla,Testudinis Carapacis et Plastri Colla and Cervi Cornus Colla.Then Tricine-SDS-PAGE electrophoresis of the obtained degradation products were performed.RESULTS Tricine-SDS-PAGE spectrum of different enzymolysis of Asini Corii Colla showed some thin strips difference for samples without enzyme treatment,and also difference for Testudinis Carapacis et Plastri Colla and Cervi Cornus Colla.CONCLUSION The method of enzymatic degradation products by Tricine-SDS-PAGE shows more information than normal PAGE,and can make a good distinction between Asini Corii Colla and other animal gelatin.