Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

Abstract Triazole‐backbone oligonucleotides are macromolecules that have one or more triazole units that are acting as a backbone mimic. Triazoles within the backbone have been used within oligonucleotides for a variety of applications. This unit describes the preparation and synthesis of two triazole‐nucleoside phosphoramidites [uracil‐triazole‐uracil (UtU) and cytosine‐triazole‐uracil (CtU)] based on a PNA‐like scaffold, and their incorporation within oligonucleotides. Curr. Protoc. Nucleic Acid Chem. 55:4.57.1‐4.57.38. © 2013 by John Wiley & Sons, Inc.