Knee joint injection resveratrol amelioration inflammation in collagen antibody induced arthritis

Background: Resveratrol has been considered to possess anti-inflammatory properties. Here, we propose that intra-articular resveratrol injection might ameliorate acute inflammation in a single knee joint in mice with collagen antibody-induced arthritis (CAIA). Methods: The clinical expression of CAIA was analyzed by two investigators. Resveratrol (30 mg/kg in 30 μL dimethyl sulfoxide [DMSO]) was injected into the knee joint of BALB/c mice from days 0 to 9. Footpad thickness was photographed and calculated. On day 10, the joints were examined histopathologically. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. mRNA levels in paws were measured by real-time polymerase chain reaction (PCR). Results: In CAIA, acute inflammation was induced on day 7 and continued until day 10. In the resveratrol-treated mice, the severity of inflammation was obviously reduced. Expression levels of interleukin-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase were decreased in the treated mice; however, tumor necrosis factor-α (TNF-α) levels did not change significantly after treatment. Conclusion: The anti-inflammatory mechanism of resveratrol is associated with NF-κB inhibition. Further investigations of the clinical applications of resveratrol are required.