硫堇
扫描电化学显微镜
电催化剂
计时安培法
佩多:嘘
电化学
水平扫描速率
材料科学
电极
化学
化学工程
纳米技术
分析化学(期刊)
循环伏安法
有机化学
物理化学
工程类
作者
Susan Warren,Grigore Munteanu,Dhanraj Rathod,Timothy McCormac,Eithne Dempsey
标识
DOI:10.1016/j.bios.2019.04.042
摘要
Herein we report the use of scanning electrochemical microscopy (SECM) together with electrochemical and spectroscopic techniques to develop and characterise a stable and uniformly reactive chemically modified platinum electrode for NADH electrocatalysis. In order to achieve this, a range of different approaches for thionine entrapment within an electropolymerised poly (3,4-ethylendioxythiophene) (PEDOT) film were evaluated using SECM imaging in the presence of NADH, demonstrating the uniformity of the reactive layer towards NADH oxidation. The effect of electrolyte type and time scale employed during PEDOT electropolymerisation was examined with respect to thionine loading and the resulting charge transport diffusion coefficient (DCT) estimated via chronoamperometry. These studies indicated a decrease in DCT as thionine loading increased within the PEDOT film, suggesting that charge transport was diffusion limited within the film. Additionally, thionine functionalised nanotubes were formed, providing a stable support for lactate dehydrogenase entrapment while lowering the rate of thionine leaching, determined via SECM imaging. This enabled lactate determination at Eapp = 0.0 V vs Ag/AgCl over the range 0.25–5 mM in the presence of 1 mM NAD+.
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