PO-166 The expression of nicotinate nucleotide pyrophosphorylase is elevated in colorectal cancer tissues

Introduction Colorectal cancer (CRC) is one of the most prevalent cancer worldwide including Korea and has poor prognosis. Recent improvement in methods for proteome analysis has offered the possibility of identifying disease-associated protein markers to assist in diagnosis or prognosis, and for selecting potential targets for specific drug therapy. Material and methods Proteomic analysis was performed with proteins from colorectal cancer (CRC) and the corresponding normal colorectal tissues, and analysed a number of proteins to isolate and identify tumor-specific proteins in CRC by two dimensional gel electrophoresis (2-DE), tryptic digestion, and MALDI-TOF/mass spectrometry. To further invetigate the targeted protein with altered expression level, western blot analysis, real-time PCR, and immunohistochemistry were performed. Results and discussions In current study, the proteome of colorectal adenocarcinoma and the corresponding normal tissues was visualised and analysed a number of proteins for CRC. Ten proteins were dominantly expressed and five proteins were largely repressed in CRC by the analysis of 2-DE and MALDI-TOF/MS. Nicotinate nucleotide pyrophosphorylase was focused to be increased in CRC tissues compared with normal colorectal tissues. The elevated expression of nicotinate nucleotide pyrophosphorylase in CRC was confirmed by western blot analysis and real time PCR. Immunohistochemistry also verified largely up-regulation of nicotinate nucleotide pyrophosphorylase in 85 CRC patients. Conclusion These findings suggest that nicotinate nucleotide pyrophosphorylase could be possible tumour biomarker candidates in CRC tissues.