IDDF2018-ABS-0023 Butyrate acid as a potential marker for the diversity of gut microbiota in colorectal cancer patients

Background

The gut microbiota turns as an actual organism, and many changes of variety have been described in colorectal cancer patients (CRC). Butyrate is a part of short chain fatty acid which commonly produced by especially bacteria in the colon, and it was to use of their host. Several kinds of literature reported the role of butyrate as prevention or inhibition of carcinogenesis of intestinal. In addition, there is an opposite the link between butyrate acid’s level and the rate of frequency CRC patients. In this study, we evaluated the level of butyrate acid as diagnostic biomarkers for the diversity of gut microbiota in colorectal cancer patients.

Methods

The study consists of fourteen subjects with CRC and 14 non-CRC were included in this case-control study. Stool samples were analysed for SCFA (acetate, propionate, and butyrate acids) with gas chromatography and the result is given as ug/mL. Especially, we determined various cut-off marks for butyrate acids in respect of indicating CRC for addition to the computation of both sensitivity and specificity.

Results

We found that CRC patients had a lower level of acetate, propionate and butyrate acids than non-CRC. The mean concentration of acetate 8,55 ug/mL, propionate 5,61 ug/mL and butyrate acids 3,79 ug/mL respectively. In three of SCFA, the level of butyrate acids had the best diagnostic properties with area under receiver operating characteristic (ROC) curve of 0.84 higher than acetate (0.71) and propionate (0.75) (p<0.05). With a cut-off value for butyrate acids<5.4 ug/mL indicating CRC, the sensitivity, specificity, positive and negative likelihood ratio, and diagnostic odds ratio were 85%, 78%, 4.04, 0.18 and 22.2 respectively.

Conclusions

In conclusion, our study is the first report demonstrating the level butyrate acids as useful biomarkers to detect the presence of CRC. Due to the small size of the sample and this design study is case-control, needs more require approval to confirm the result.