机械生物学
微流控
间充质干细胞
材料科学
生物材料
纳米技术
海藻酸钙
细胞
细胞培养
自愈水凝胶
细胞内
生物医学工程
生物物理学
化学
细胞生物学
钙
生物
工程类
高分子化学
冶金
生物化学
遗传学
作者
Berna Özkale,Junzhe Lou,Ece Özelçi,Alberto Elósegui-Artola,Christina M. Tringides,Angelo S. Mao,Mahmut Selman Sakar,David J. Mooney
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:22 (10): 1962-1970
被引量:5
摘要
We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optically triggered nanoactuator-alginate hybrid biomaterial using microfluidics, and the encapsulating network isotropically compresses the cell upon activation by light. The favorable biomolecular properties of alginate allow cell culture in vitro up to a week. The mechanically active microgels are capable of generating up to 15% compressive strain and forces reaching 400 nN. As a proof of concept, we demonstrate the use of the mechanically active cell culture system in mechanobiology by subjecting singly encapsulated MSCs to optically generated isotropic compression and monitoring changes in intracellular calcium intensity.
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