A pH-applicative fluorescent probe with long measuring range for monitoring hydrazine in water samples and Arabidopsis thaliana

A fluorescent probe, RhodCl-Hz , with pH-applicative capability and long measuring range, was developed to serve the topic on the enrichment process of hydrazine. It was practical due to the clear acid-base boundary and the sectioned linear ranges. With the excitation wavelength of 515 nm and the emission peak at 565 nm, the detecting system was steady. It exhibited a clear cut-off point at pH 7.0 and steady fluorescence signals within the range of 7.0–10.0. As a whole, the linear range of 10.0–500 µM was long. With high sensitivity and selectivity, RhodCl-Hz was applied to suit water samples and biological imaging in both Arabidopsis Thaliana root tips and living MCF-7 cells. • A pH-applicative fluorescent probe for imaging hydrazine, RhodCl-Hz . • The linear range of 10.0–500 µM was long for practical use. • High sensitivity and high selectivity towards hydrazine. • Applied in the imaging in cells and Arabidopsis thaliana. In this work, a fluorescent probe, RhodCl-Hz , with pH-applicative capability and long measuring range, was developed to serve the topic on the enrichment process of hydrazine. It was practical due to the clear acid-base boundary and the sectioned linear ranges. With the excitation wavelength of 515 nm and the emission peak at 565 nm, the detecting system was steady. It exhibited a clear cut-off point at pH 7.0 and steady fluorescence signals within the range of 7.0–10.0. As a whole, the linear range of 10.0–500 µM (1.0–50.0 equivalent) was long. The Limit of Detection value was calculated as 0.64 μM. With high selectivity, RhodCl-Hz was applied to suit water samples and biological imaging in both Arabidopsis Thaliana root tips and living MCF-7 cells. The information here might be helpful for revealing the enrichment process of hydrazine.