Screening of specific quantitative peptides of beef by LC–MS/MS coupled with OPLS-DA

A rapid, simple, and efficient analysis methodology for screening specific quantitative peptides of beef was established based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with orthogonal partial least squares-discriminant analysis (OPLS-DA). The OPLS-DA model was built to select species-specific peptides that make a significant contribution to classification. Peptides with statistical significance were selected based on the variable importance in the projection (VIP) values and univariate P values. After the workflow of the statistical process, three specific quantitative peptides were identified by using homemade products with different beef contents. A quantification method for selected specific quantitative peptides was established by using LC-MS/MS. The quantitative results were applied to commercialized beef products. The developed method has high sensitivity, specificity, and repeatability. The results of this study proved that the integration of LC-MS/MS coupled with OPLS-DA is an efficient method for screening specific quantitative peptides and identification of the authenticity of meat products.