Turbidimetric Assay for Pectinase Activity

Abstract A simple procedure for the estimation of polygalacturonic acid and polymethyl‐galacturonic acid hydrolases is described. The method is based on measuring the residual turbidity formed by complexing the acid polysaccharide with quaternary‐ammonium detergents such as cetyl pyridinium bromide. The assay is carried out at pH 4.5 to 5.0 which is optimal for the hydrolase activities.