HPLC analysis of polyamines and their acetylated derivatives in the picomole range using benzoyl chloride and 3,5‐dinitrobenzoyl chloride as derivatizing agent

Abstract Analytical methods are described for the quantitative determination of putrescine, cadaverine, spermidine, spermine and the acetylated derivatives of spermidine and spermine in biological fluids using pre‐column derivatization with either benzoyl chloride or 3,5‐dinitrobenzoyl chloride, which were added to each sample as solutions in diethyl ether. Putrescine, spermidine and spermine can be analysed in seminal plasma at nanogram levels when benzoyl chloride is used as derivatizing agent. In the analysis of putrescine, cadaverine, spermidine and acetyl derivatives of spermidine and spermine, higher sensitivity is obtained with 3,5‐dinitrobenzoyl‐chloride. This method can readily be used in the determination of acetylated polyamines in urine samples.