葡萄糖氧化酶
固定化酶
辣根过氧化物酶
催化作用
化学
等温微量热法
多孔性
化学工程
基质(水族馆)
介孔材料
微型多孔材料
色谱法
材料科学
酶
有机化学
物理
海洋学
量子力学
工程类
焓
地质学
作者
Huibin Pan,Xia Gao,Mei Chen,Le Su,Chengfang Qiao,Jun Wang,Chunsheng Zhang,Jiu-Fu Lu,Hongguang Ge
标识
DOI:10.1016/j.bej.2023.109161
摘要
Metal-organic frameworks (MOFs) are considered as promising carriers for immobilized enzymes. Whereas, some interrelated and restrictive problems exist in the construction of MOFs immobilized enzymes with excellent catalytic performance, resulting in reduced catalytic activity of the enzymes. In this work, a novel hierarchical porous structure of Zr-based metal-organic frameworks (Zr-MOF, HPU) was accurately constructed for co-immobilizing horseradish peroxidase (HRP) and glucose oxidase (GOx). The prepared HPU carrier can enrich the substrate by using the abundant micropore structure around the mesopore, thus reducing the mass transfer resistance and making up for the loss of enzyme activity during enzyme immobilization. Notably, GOx-HRP@HPU demonstrated outstanding catalytic efficiency and excellent stability, as well as ideal reusability, maintaining nearly 95% initial activity at 60 ℃ after 1 h incubation and above 65% initial activity after 10 reuses. Both the immobilization process and interaction of substrates with co-immobilized bienzyme reactor were investigated by means of microcalorimetry, which demonstrated HPU was an excellent platform for co-immobilized HRP and GOx. Furthermore, GOx-HRP@HPU was used for the detection of glucose with excellent detection performance.
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