清脆的
基因组编辑
Cas9
计算生物学
生物
基因
计算机科学
生物技术
遗传学
作者
Junming Zhou,Xinchao Luan,Yixuan Liu,Lixue Wang,Jiaxin Wang,Songnan Yang,Shuying Liu,Jun Zhang,Huijing Liu,Dan Yao
出处
期刊:Plants
[MDPI AG]
日期:2023-03-28
卷期号:12 (7): 1478-1478
被引量:1
标识
DOI:10.3390/plants12071478
摘要
Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has become increasingly mature and is being widely used for crop improvement. The application of CRISPR/Cas9 enables the generation of transgene-free genome-edited plants in a short period and has the advantages of simplicity, high efficiency, high specificity, and low production costs, which greatly facilitate the study of gene functions. In plant molecular breeding, the gene-editing efficiency of the CRISPR-Cas9 system has proven to be a key step in influencing the effectiveness of molecular breeding, with improvements in gene-editing efficiency recently becoming a focus of reported scientific research. This review details strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding, including Cas9 variant enzyme engineering, the effect of multiple promoter driven Cas9, and gRNA efficient optimization and expression strategies. It also briefly introduces the optimization strategies of the CRISPR/Cas12a system and the application of BE and PE precision editing. These strategies are beneficial for the further development and optimization of gene editing systems in the field of plant molecular breeding.
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