斑马鱼
心脏毒性
活性氧
发育毒性
生物
毒性
药理学
蛋白激酶B
转基因
细胞凋亡
细胞生物学
癌症研究
磷酸化
内科学
生物化学
基因
医学
遗传学
妊娠期
怀孕
作者
Junho Park,Taeyeon Hong,Garam An,Hahyun Park,Gwonhwa Song,Whasun Lim
标识
DOI:10.1016/j.scitotenv.2022.160761
摘要
Various types of fungicides, especially triazole fungicides, are used to prevent fungal diseases on farmlands. However, the developmental toxicity of one of the triazole fungicides, triadimenol, remains unclear. Therefore, we used the zebrafish animal model, a representative toxicological model, to investigate it. Triadimenol induced morphological alterations in the eyes and body length along with yolk sac and heart edema. It also stimulated the production of reactive oxygen species and expression of inflammation-related genes and caused apoptosis in the anterior regions of zebrafish, especially in the heart. The phosphorylation levels of Akt, ERK, JNK, and p38 proteins involved in the PI3K and MAPK pathways, which are important for the development process, were also reduced by triadimenol. These changes led to malformation of the heart and vascular structures, as observed in the flk1:eGFP transgenic zebrafish models and a reduction in the heart rate. In addition, the expression of genes associated with cardiac and vascular development was also reduced. Therefore, we elucidated the mechanisms associated with triadimenol toxicity that leads to various abnormalities and developmental toxicity in zebrafish.
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