To cope with the problem that the less-than-truckload(LTL) logistics network design for the certain Origin-Destination(OD) flow demand often leads to an unbalanced use of network resources,the characteristics of the uncertainty of OD flow among LTL logistics nodes and the network design difficulties brought about by the uncertain OD demand flow in LTL logistics system are analyzed,and a mixed integer programming model is established to adapt to the uncertain OD demand flow based on inventory theory.The examples demonstrate that(1) the design of logistics network with uncertain OD demand flow by optimizing the cost of transport and construction of the network as well as the utilization rate of network sources comprehensively has an obvious advantage in a balanced usage of network resources over the one only considering minimizing the cost of transport and construction of the network;(2) the result is valuable to adjust the structure of logistics network to achieve a balanced use of network resources in the case of uncertainty OD demand flow.