Establishment and application of a method using ~3H-GABA to detect γ-aminobutyric acid receptor

Purpose:to introduce a method for examination of γ-aminobutyric acid (GABA) receptor in rat's brain.Methods: 3H-GABA was used as ligand to examine GABA in microsac of rat's brain.The best acting factors were 1.0×10 -3 mol/L protein of rat's microsac acted for 30 min under 37℃.Results:GABA receptor was found in rat's brain.Kd=(97.3±5.8)×10 -9 mol/L,B max =0.1061×10 -9 mol/g protein±0.0103×10 -9 mol/g protein.It was a low-affinity class GABA receptor.Conclusion: 3H-GABA can be used for examination of GABA receptor in rat's brain.