Determination of Growth Curve of Serotype 5 of Staphylococcus aureus

[Objective] The study was conducted to determine the growth curve of serotype 5 of Staphylococcus aureus.[Method] With different mediums and culture modes,the absorption value of bacterium fluid was determined with spectrophotometer,and the growth curve of Staphylococcus aureus was drawn.[Result] In Columbia culture medium,the liquid medium more favored to the growth of Staphylococcus aureus than solid medium under shaking condition,and it was worst under stationary condition.In Mod 110 culture medium,the growing speed of Staphylococcus aureus was as follows: solidliquid(shaking condition)liquid(stationary condition).With the same culture pattern,Mod 110 medium was better than Columbia medium to the growth of Staphylococcus aureus.[Conclusion] The growth curve reflected growing trend of the bacterium,indicating that the bacterium could be researched for long term.