Objective To investigate the infection status of genitourinary tract mycoplasma in the undergraduate smaents,and to analyse the risx factors for gentournary tract mycoplasma infection,in order to provide the scientific theoretical basis of prevention against genitourinary tract mycoplasma infection in universities. Methods Questionnaire was made in order to investigate the risk factor for genitourinary tract mycoplasma infection.The objects included all students of grade 2005 in the medical college of a university.500 μl middle urine of objects was taken into the culture medium that was made by ourselves.The result was judged according to the color change of the culture.Those whose urine in the culture became red were positive(+).Others were negative(-).Every object was tested two times.Only those who were positive for two times were really positive.SPSS 13.00 was applied to statistical analysis.χ2 test was used for single factor analysis,Group Logistic Regression analysis was used for multiple factor analysis.Results 65 cases among 998 undergraduate students were mycoplasma infection positive(54 female,11 male),and the infection rate was 6.51%.Gender and swimming was found related to genitourinary tract mycoplasma infection after single factor analysis.Group Logistic regression analysis showed that sex,parents to be alive or not and family residence are risk factors.Conclusions There exists genitourinary tract mycoplasma infection in undergraduate students,and the infection rate of female is significantly higher than that of male.Gender,Parents to be alive or not and family residence are risk factors for genitourinary tract mycoplasma infection in university students.