Tissue-cultured Gymnema sylvestre Leaf Extract Enhance Anti-adipogenic Effects through Inhibition of C/EBP-${\alpha}$ Expression and Lipid Droplet Accumulation

Gymnema sylvestre, a traditional India medicine called a 'Gagaimogouip' in Korea, has been used to regu- late sugar metabolism in patients with diabetes mellitus. First, we induced callus from Gymnema sylvestre leaf and mode extract from cultured callus. In this study, we investigated the anti-obesity effect of tissue-cultured Gymnema sylvestre leaf extract in 3T3-L1 cells. The tissue-cultured Gymnema sylvestre extract inhibited the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocytes. The tissue-cultured Gymnema sylvestre extract inhibited insulin-induced predipocyte differentiation through the inhibition of expression of the early adipogenic tran- scription factor C/EBP-α that regulate adipogenesis. These results indicate that tissue-cultured Gymnema sylvestre extract may be potential natural ingredient for slimming cosmetic products.