Studies on Compound Mutagenesis Method of Protoplast of Mortierella Ramanniana Obtaining High γ-Linolenic Acid Yield Strain

A mutant strain M_(F10) accumulating a large amount of lipid was successfully obtained when the original strain Morlicrella ramanniana-M_5 was treated by compound mutagenesis through the use of HNO_2 and laser after its cell walls was decomposed.The content of lipid in M_(F10) mycelium culture in shaking flask with its all dry cell weight was up to 46.8 g/L which is 173%higher than that in the original strain,and its lipid yield 34.5 g/L andγ-Linolenic acid yield 12.5 g/L are 202%and 260%higher than its original strain.The passing generation lest showed that the heredity character of mutant M_(F10) was stable.