Enzymatic Hydrolysis of Silk Fibroin for Production of ACE Inhibitors

Silk fibroin was hydrolyzed by Alcalase to produce the peptide with high ACE-inhibition activity.The effects of several influential factors on the enzymatic hydrolysis were investigated and the optimum reaction conditions were determined as follows: pH 8.5,temperature 60 ℃,substrate concentration 5%,enzyme dosage 1000 U/g protein,and reaction time 240 min,under which the DH,ACE-inhibition rate,peptide yield and protein recovery of the product were17.12%,74.07%,50.31% and 77.37%,respectively.MALDI-TOF mass spectrometry of the hydrolysate showed that the molecular weight of the peptides was below 2.4kDa.