Optimizing of RAPD amplifying condition and analysis of genetic DNA polymorphisms of plants of Dendrobium

Objective: The optimal reaction of RAPD(random amplified polymorphic DNA)of Dendrobium nobile was set up and it was used in determing of plants of Dendrobium DNA polymorphisms.Methods: The different concentration were analyzed from template DNA,Mg2+,Taq DNA polymerases,dNTPs,primeron RAPD amplification. Results: In 25 μl RAPD reaction system those concentration were 100 ng,3.0 mmol/L,1.0 U,0.2 mmol/L,0.4 μmol/L respectively,and the DNA fringerprintings of Dendrobium were distinct image,reliable stability and good repeatability.The DNA fingerprints amplified with the primer S48 had a similarity,the type of fingerprints still more suited the discrimination of true and fasle Dendrobium;but The fragments of amplifying the genetic DNA of Dendrobium with the primer S90 had more polymorphisms.The type of DNA fingerprints still more suited the study of genetic divergence in Dendrobium. Conclusion: The optimizing RAPD technique was effective in studying genetic divergence in Dendrobium and characterizing species of Dendrobium and its adulterant specise.