PAGE(polyacrylamide gel electrophoresis) and saturation ammonium sulfate sedimentation were used to analyze serum proteins of three sturgeons,2~+ year old Amur sturgeon Acipenser schrenckii Brandt,3~+ year old Chinese sturgeon Acipenser sinesis Gray and 3 years old Huso Beluga.Chinese sturgeon,Amur sturgeon,and Huso Beluga had total serum protein level of 18.70 mg/mL,21.25 mg/mL and 24.60 mg/mL,respectively.Relationship coefficient of serum protein showed that Amur sturgeon was more closer to Chinese sturgeon than to Huso Beluga.There were 8 bands in Chinese sturgeon,13 bands in Amur sturgeon and 8 bands in Huso Beluga by PAGE.