Role of Reproductive Hormone and LDH in the Expression of Apoptosis Protein in Spermatogenic Cell of Rats Exposed to Manganese

Objective The purpose was to study the expression of caspase-3 in spermatogenic cells and the contents of reproductive hormone,lactate dehydrogenase( LDH) of Rats Exposed to Manganese and to discuss the relationship between them. Method 48 male rats weighting( 120 ± 10) g were randomly divided into 6 groups( blank control,15 mg / kg and 30 mg / kg MnCl 2),8 rats/group. MnCl 2 groups were contaminated manganese( MnCl 2 ·4H 2 O) by I. P. for 4 and 6 weeks respectively and blank control groups was injected with NS. All rats were injected once a day,five times per week. In the 4th and 6th weekend,their testis and serum were collected. The expression of caspase-3 in testis was investigated by immunohisto chemical methods,and the contents of T,FSH and LH in serum were measured by radioimmu noassay,the activity of LDH in serum and testis were measured by colorimetric analysis. Results Compared with blank control groups,the caspase-3-positive-cell rate was increased,the contents of T was decreased but FSH and LH were increased in every manganese exposure group( P 0. 01),the activity of LDH was increased in serum in 6 w 15 mg / kg group,and decreased in testis in every MnCl 2 groups( P 0. 01). Among manganese exposure groups of same dose,the caspase-3-positive-cell rate and the contents of FSH,LH in serum were increased,the activity of LDH was increased in serum but decreased in testis significantly in 6 w MnCl 2 groups than it in 4 w groups( P 0. 01). Among manganese exposure groups of same time,the caspase-3-positive-cell rate and the contents of FSH,LH in serum were increased,the activity of LDH was increased in serum but decreased in testis in 30 mg / kg manganese exposure groups than them in 15 mg / kg groups( P 0. 01). Conclusion MnCl 2 at dose of 15mg / kg can decrease the contents of T,increase the contents of FSH,LH in serum,and decrease the activity of LDH in testis and increase it in serum,and they might be the important causes of accelerating the expression of caspase-3 in rat spermatogenic cells.