Effects of stretch receptor ablation on the optomotor control of lift in the hawkmothManduca sexta

SUMMARY In insects, fast sensory feedback from specialized mechanoreceptors is integrated with guidance cues descending from the visual system to control flight behavior. A proprioceptive sensory organ found in both locusts and moths, the wing hinge stretch receptor, has been extensively studied in locusts for its powerful influence on the activity of flight muscle motoneurons and interneurons. The stretch receptor fires a high-frequency burst of action potentials near the top of each wingstroke and encodes kinematic variables such as amplitude and timing. Here, I describe the effects of stretch receptor ablation on the visual control of lift during flight in the hawkmoth Manduca sexta. Using a combination of extracellular muscle recordings, force and position measurements and high-speed video recording, I tracked power muscle activity, net vertical flight force (lift), abdomen deflection and wing kinematics in response to image motions of varying velocity during tethered flight in a wind tunnel. As a result of bilateral ablation of the wing hinge stretch receptors, visually evoked lift decreased to nearly one-third of that exhibited by intact animals. The phase and frequency of indirect power muscle action potentials and the patterns of abdominal deflection were unaffected; however, wingstroke amplitude was clearly reduced after ablation. Collectively, these results suggest that stretch receptor feedback is integrated with descending visual cues to control wing kinematics and the resultant aerodynamic force production during flight.