生物
癌症研究
磷酸化
长非编码RNA
癌症
转移
原癌基因酪氨酸蛋白激酶Src
酪氨酸激酶
细胞生物学
受体酪氨酸激酶
信号转导
下调和上调
基因
遗传学
作者
Hui Zhang,Ran‐Ran Ma,Guo‐Hao Zhang,Yi Dong,Miao Duan,Yiyuan Sun,Yaru Tian,Jianlu Gao,Xu Chen,Haiting Liu,Peng Gao
标识
DOI:10.1016/j.canlet.2021.09.042
摘要
Long non-coding RNAs (lncRNAs) play important roles in cancer development and progression; however, their contributions to gastric cancer metastasis remain largely unknown. By lncRNA microarray screening, our study showed that 453 lncRNAs are dysregulated in gastric cancer tissues with or without lymph node metastasis, of which lnc-LEMGC ranks as one of the most significantly downregulated lncRNAs. Lnc-LEMGC inhibited cell migration and invasion both in vitro and in vivo, by combining with protein DNA-PKcs. Importantly, nucleotides 1300-1800 of lnc-LEMGC prevented DNA-PKcs phosphorylation of serine 2056 and partially abrogated the effects of downstream effectors, ErbB1, SRC and protein tyrosine kinase 2 (FAK), in the epidermal growth factor receptor (EGFR) pathway. The results of this study extend our knowledge of lncRNA's molecular mechanisms, in which lnc-LEMGC functions by directly suppressing the phosphorylation of its combined protein DNA-PKcs and inactivating the DNA-PKcs downstream EGFR signaling.
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