脂质过氧化
活性氧
生物
多不饱和脂肪酸
生物化学
电离辐射
细胞凋亡
细胞
癌细胞
氧化应激
细胞生物学
辐照
脂肪酸
癌症
遗传学
物理
核物理学
作者
Chao Mao,Guang Lei,Amber Horbath,Boyi Gan
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2022-01-01
卷期号:: 37-50
标识
DOI:10.1016/bs.mcb.2022.05.003
摘要
Lipid peroxidation occurs under conditions where reactive oxygen species (ROS) readily react with vulnerable lipids on cell membranes. Polyunsaturated fatty acids (PUFAs) are highly susceptible to lipid peroxidation because of their unstable double bonds. Because the cell membrane is particularly rich in PUFAs, it is often the site at which many lipid peroxidation chain reactions occur. Lipid peroxidation is considered the ultimate trigger of ferroptosis, an iron-dependent form of non-apoptotic cell death. Radiotherapy is a common cancer treatment that uses high-energy ionizing radiation to kill cancer cells, and radiation-induced cell death is partially attributed to lipid peroxidation-driven ferroptosis. Here, we describe methods to assess lipid peroxidation in irradiated cells. The same techniques can be applied to a variety of lipid peroxidation measurements under different treatment conditions.
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