A four-pool, in vivo kinetic model (blood glucose and CO2 and rumen propionate and CO2) was proposed for propionate and glucose metabolism in Holstein steers. Two daily perturbations were 6.1 mol dietary propionate and 1.18 mol glucose excreted. Calculated solutions indicated 6.6 mol propionate added and 1.10 mol glucose excreted. Key words: Propionate, glucose, kinetics, modeling, cattle