Studies of silver staining of human parotid saliva protein polyacrylamide gel electrophoretograms

Abstract The silver staining procedure of Oakley et al. [3] has been applied to parotid saliva proteins and certain protein standards fractionated by isoelectric focusing and electrophoresis in denaturing and non‐denaturing polyacrylamide gels. This staining method was up to 100‐fold more sensitive than Coomassie Blue staining for sodium dodecyl sulphate‐containing gels and for focusing gels, after prefixation. In non‐denaturing gels, however, the relative sensitivity ( i.e. silver vs. Coomassie) of different bands varies widely but this can be overcome by silver staining of gels after Coomassie Blue staining.