替莫唑胺
介孔二氧化硅
细胞凋亡
胶质瘤
肽核酸
化学
细胞培养
材料科学
生物物理学
癌症研究
核酸
生物化学
介孔材料
生物
遗传学
催化作用
作者
Alessandro Bertucci,Eko Adi Prasetyanto,Dedy Septiadi,Alex Manicardi,Eleonora Brognara,Roberto Gambari,Roberto Corradini,Luisa De Cola
出处
期刊:Small
[Wiley]
日期:2015-09-23
卷期号:11 (42): 5687-5695
被引量:121
标识
DOI:10.1002/smll.201500540
摘要
Mesoporous silica nanoparticles (MSNPs), 100 nm in size, incorporating a Cy5 fluorophore within the silica framework, are synthesized and loaded with the anti-cancer drug temozolomide (TMZ), used in the treatment of gliomas. The surface of the particles is then decorated, using electrostatic interactions, with a polyarginine-peptide nucleic acid (R8-PNA) conjugate targeting the miR221 microRNA. The multi-functional nanosystem thus obtained is rapidly internalized into glioma C6 or T98G cells. The anti-miR activity of the PNA is retained, as confirmed by reverse transcription polymerase chain reaction (RT-PCR) measurements and induction of apoptosis is observed in temozolomide-resistant cell lines. The TMZ-loaded MSNPs show an enhanced pro-apoptotic effect, and the combined effect of TMZ and R8-PNA in the MSNPs shows the most effective induction of apoptosis (70.9% of apoptotic cells) thus far achieved in the temozolomide-resistant T98G cell line.
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