纯化蛋白衍生物
抗原
结核菌素
牛分枝杆菌
肺结核
结核分枝杆菌
蛋白质组学
生物
化学
微生物学
分子生物学
生物化学
免疫学
医学
基因
病理
作者
Sibele Borsuk,Jane Newcombe,Tom A. Mendum,Odir A. Dellagostin,Johnjoe McFadden
出处
期刊:Tuberculosis
[Elsevier]
日期:2009-11-01
卷期号:89 (6): 423-430
被引量:58
标识
DOI:10.1016/j.tube.2009.07.003
摘要
The tuberculin purified protein derivative (PPD) is a widely used diagnostic antigen for tuberculosis, however it is poorly defined. Most mycobacterial proteins are extensively denatured by the procedure employed in its preparation, which explains previous difficulties in identifying constituents from PPD to characterize their behaviour in B- and T-cell reactions. We here described a proteomics-based characterization of PPD from several different sources by LC-MS/MS, which combines the solute separation power of HPLC, with the detection power of a mass spectrometer. The technique is able to identify proteins from complex mixtures of peptide fragments. A total of 171 different proteins were identified among the four PPD samples (two bovine PPD and two avium PPD) from Brazil and UK. The majority of the proteins were cytoplasmic (77.9%) and involved in intermediary metabolism and respiration (24.25%) but there was a preponderance of proteins involved in lipid metabolism. We identified a group of 21 proteins that are present in both bovine PPD but were not detected in avium PPD preparation. In addition, four proteins found in bovine PPD are absent in Mycobacterium bovis BCG vaccine strain. This study provides a better understanding of the tuberculin PPD components leading to the identification of additional antigens useful as reagents for specific diagnosis of tuberculosis.
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