多糖
皂甙
色谱法
化学
山茶花
洗脱
吸附
解吸
萃取(化学)
山茶
柱色谱法
植物
有机化学
生物
病理
替代医学
医学
作者
Penghui Yang,Mingda Zhou,Chengyun Zhou,Qian Wang,Fangfang Zhang,Jian Chen
标识
DOI:10.1002/jssc.201401123
摘要
A novel method to separate and purify tea seed polysaccharide and tea seed saponin from camellia cake extract by macroporous resin was developed. Among four kinds of resins (AB-8, NKA-9, XDA-6, and D4020) tested, AB-8 macroporous resin possessed optimal separating capacity for the two substances and thus was selected for the separation, in which deionized water was used to elute tea seed polysaccharide, 0.25% NaOH solution to remove the undesired pigments, and 90% ethanol to elute tea seed saponin. Further dynamic adsorption/desorption experiments on AB-8 resin-based column chromatography were conducted to obtain the optimal parameters. Under optimal dynamic adsorption and desorption conditions, 18.7 and 11.8% yield of tea seed polysaccharide and tea seed saponin were obtained with purities of 89.2 and 96.0%, respectively. The developed method provides a potential approach for the large-scale production of tea seed polysaccharide and tea seed saponin from camellia cake.
科研通智能强力驱动
Strongly Powered by AbleSci AI