PENGGUNAAN XILANASE Streptomyces sp. 45 I-3 AMOBIL UNTUK HIDROLISIS XILAN TONGKOL JAGUNG [Immobilization of Extracellular Xylanase from Streptomyces sp. 45 I-3 for Hydrolysis of Corncob Xylan ]
作者
Titi Candra Sunarti,Ferry Mutia,Niken Financia Gusmawati,Yulin Lestari,Anja Meryandini
出处
期刊:Jurnal Teknologi dan Industri Pangan [Bogor Agricultural University; Indonesian Food Technologist Association (IAFT)] 日期:2009-06-13卷期号:20 (1): 9-9
Xylan extraction from corncob is done by using alkaline as solvent. Xylan extraction from corncob could give the yields as 10.9%. One percent of corncob xylan is used as substrate to produce the xylanase, compared to oatspelt xylan. Immobilization of xylanase was performed using 1% Eudragit TM S100 solution (w/v), with 5:1 volume ratio of xylanase and 1 % Eudragit TM S100 (w/v). Activity of the immobilized xylanase was decreased to 23.97% compared with free xylanase. Immobilized xylanase have optimum pH and temperature at 6.0 and 40 ° C respectively, have also thermal stability at 30–40 ° C for an hour. Immobilized xylanase could be reused, but its activity decreased to 52.38% after 3 times application.