Efficacy of a therapeutic treatment using gas‐filled microbubble‐associated phospholipase A2 in a mouse model of honeybee venom allergy

Abstract Background Venom immunotherapy is efficient to desensitize people suffering from insect sting allergies. However, the numerous injections required over several years and important risks of severe side reactions complicate the widespread use of immunotherapy. In the search for novel approaches to blunt the overwhelming pro‐allergic Th2 response, we evaluated the therapeutic efficacy of a treatment based on a denatured form of the major allergen, phospholipase A2, associated with microbubbles ( PLA 2 denat ‐ MB ) in a mouse model of honeybee venom allergy. Methods Antibodies measured by ELISA , T‐cell responses assessed by CFSE ‐based proliferation assays and ELISA , and basophil degranulation were examined after PLA 2 denat ‐ MB ‐based therapeutic treatment of sensitized mice. Mice were challenged with a lethal dose of PLA 2 to evaluate protection against anaphylaxis. Results Therapeutic subcutaneous administration of two different PLA 2 denat ‐ MB formulations, in contrast to PLA 2 denat alone, reduced allergic symptoms and protected all mice from anaphylaxis‐mediated death after allergen challenge. At the functional level, the use of PLA 2 denat decreased IgE‐mediated basophil degranulation as compared to the native form of the allergen. In comparison with PLA 2 denat alone, both PLA 2 denat ‐ MB formulations decreased allergen‐specific Th2 CD 4 T‐cell reactivity. At the mechanistic level, PLA 2 denat ‐ MB containing 20% palmitic acid and PEG induced PLA 2‐specific IgA and increased Foxp3 + Treg frequencies and TGF ‐β production, whereas the formulation bearing 80% palmitic acid triggered the production of IFN ‐γ, IgG2a, and IgG3. Conclusions In contrast to conventional PLA 2 subcutaneous immunotherapy, the therapeutic administration of PLA 2‐ MB treatment to mice that already had established allergy to PLA 2 protects all subsequently challenged animals.